Part:BBa_K3882002:Experience

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Applications of BBa_K3882002

How to use it? E. bsuahlteminator bio-brick can transform to any competent bacterial. We use E. Coli BL21 as a recipe and safe bacterial to do the transformation. We use the LB media to culture the E. bsuahlteminator. When the OD value reach to 0.8, we add 200 uM IPTG into the LB media to activate the E. bsuahlteminator bio-brick inside at 30 degree centigrade for 4 hrs. After that, we use protein purification kit from Beyotime company, followed by detecting the concentration of PVDQGFP. The protein can be directly added into the LB media and spray on the surface of LB-agar plate as well as shrimp, meat. It has an inhibitory function on P. aeruginosa growth and can last for at least 5-8 hrs.

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